Expression profiling across wild and cultivated tomatoes supports the relevance of early miR482/2118 suppression for Phytophthora resistance.
Identifieur interne : 000779 ( Main/Exploration ); précédent : 000778; suivant : 000780Expression profiling across wild and cultivated tomatoes supports the relevance of early miR482/2118 suppression for Phytophthora resistance.
Auteurs : Sophie De Vries [Canada, Allemagne] ; Andreas Kukuk [Allemagne] ; Janina K. Von Dahlen [Allemagne] ; Anika Schnake [Allemagne] ; Thorsten Kloesges [Allemagne] ; Laura E. Rose [Allemagne]Source :
- Proceedings. Biological sciences [ 1471-2954 ] ; 2018.
Descripteurs français
- KwdFr :
- MESH :
- génétique : ARN des plantes, Lycopersicon esculentum, Maladies des plantes, Résistance à la maladie, microARN.
- microbiologie : Lycopersicon esculentum, Maladies des plantes.
- physiologie : Phytophthora.
- Coévolution biologique.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : MicroRNAs, RNA, Plant.
- genetics : Disease Resistance, Lycopersicon esculentum, Plant Diseases.
- microbiology : Lycopersicon esculentum, Plant Diseases.
- physiology : Phytophthora.
- Biological Coevolution.
Abstract
Plants possess a battery of specific pathogen resistance (R-)genes. Precise R-gene regulation is important in the presence and absence of a pathogen. Recently, a microRNA family, miR482/2118, was shown to regulate the expression of a major class of R-genes, nucleotide-binding site leucine-rich repeats (NBS-LRRs). Furthermore, RNA silencing suppressor proteins, secreted by pathogens, prevent the accumulation of miR482/2118, leading to an upregulation of R-genes. Despite this transcriptional release of R-genes, RNA silencing suppressors positively contribute to the virulence of some pathogens. To investigate this paradox, we analysed how the regulation of NBS-LRRs by miR482/2118 has been shaped by the coevolution between Phytophthora infestans and cultivated and wild tomatoes. We used degradome analyses and qRT-PCR to evaluate and quantify the co-expression of miR482/2118 and their NBS-LRR targets. Our data show that miR482/2118-mediated targeting contributes to the regulation of NBS-LRRs in Solanum lycopersicum. Based on miR482/2118 expression profiling in two additional tomato species-with different coevolutionary histories with P. infestans-we hypothesize that pathogen-mediated RNA silencing suppression is most effective in the interaction between S. lycopersicum and P. infestans Furthermore, an upregulation of miR482/2118 early in the infection may increase susceptibility to P. infestans.
DOI: 10.1098/rspb.2017.2560
PubMed: 29491170
PubMed Central: PMC5832704
Affiliations:
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Rose</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Population Genetics, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf, Germany laura.rose@hhu.de.</nlm:affiliation><country wicri:rule="url">Allemagne</country><wicri:regionArea>Institute of Population Genetics, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Düsseldorf</region><settlement type="city">Düsseldorf</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>iGRAD-Plant Graduate School, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>iGRAD-Plant Graduate School, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Düsseldorf</region><settlement type="city">Düsseldorf</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Ceplas, Cluster of Excellence in Plant Sciences, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Ceplas, Cluster of Excellence in Plant Sciences, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Düsseldorf</region><settlement type="city">Düsseldorf</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:29491170</idno><idno type="pmid">29491170</idno><idno type="doi">10.1098/rspb.2017.2560</idno><idno type="pmc">PMC5832704</idno><idno type="wicri:Area/Main/Corpus">000816</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000816</idno><idno type="wicri:Area/Main/Curation">000816</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000816</idno><idno type="wicri:Area/Main/Exploration">000816</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Expression profiling across wild and cultivated tomatoes supports the relevance of early miR482/2118 suppression for <i>Phytophthora</i> resistance.</title><author><name sortKey="De Vries, Sophie" sort="De Vries, Sophie" uniqKey="De Vries S" first="Sophie" last="De Vries">Sophie De Vries</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Canada NS B3H 4R2 sophie.devries@dal.ca.</nlm:affiliation><country wicri:rule="url">Canada</country><wicri:regionArea>Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax</wicri:regionArea><wicri:noRegion>Halifax</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:affiliation>Institute of Population Genetics, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Population Genetics, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Düsseldorf</region><settlement type="city">Düsseldorf</settlement></placeName></affiliation></author><author><name sortKey="Kukuk, Andreas" sort="Kukuk, Andreas" uniqKey="Kukuk A" first="Andreas" last="Kukuk">Andreas Kukuk</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Population Genetics, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Population Genetics, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Düsseldorf</region><settlement type="city">Düsseldorf</settlement></placeName></affiliation></author><author><name sortKey="Von Dahlen, Janina K" sort="Von Dahlen, Janina K" uniqKey="Von Dahlen J" first="Janina K" last="Von Dahlen">Janina K. Von Dahlen</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Population Genetics, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Population Genetics, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Düsseldorf</region><settlement type="city">Düsseldorf</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>iGRAD-Plant Graduate School, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>iGRAD-Plant Graduate School, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Düsseldorf</region><settlement type="city">Düsseldorf</settlement></placeName></affiliation></author><author><name sortKey="Schnake, Anika" sort="Schnake, Anika" uniqKey="Schnake A" first="Anika" last="Schnake">Anika Schnake</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Population Genetics, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Population Genetics, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Düsseldorf</region><settlement type="city">Düsseldorf</settlement></placeName></affiliation></author><author><name sortKey="Kloesges, Thorsten" sort="Kloesges, Thorsten" uniqKey="Kloesges T" first="Thorsten" last="Kloesges">Thorsten Kloesges</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Population Genetics, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Population Genetics, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Düsseldorf</region><settlement type="city">Düsseldorf</settlement></placeName></affiliation></author><author><name sortKey="Rose, Laura E" sort="Rose, Laura E" uniqKey="Rose L" first="Laura E" last="Rose">Laura E. Rose</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Population Genetics, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf, Germany laura.rose@hhu.de.</nlm:affiliation><country wicri:rule="url">Allemagne</country><wicri:regionArea>Institute of Population Genetics, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Düsseldorf</region><settlement type="city">Düsseldorf</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>iGRAD-Plant Graduate School, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>iGRAD-Plant Graduate School, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Düsseldorf</region><settlement type="city">Düsseldorf</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Ceplas, Cluster of Excellence in Plant Sciences, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Ceplas, Cluster of Excellence in Plant Sciences, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Düsseldorf</region><settlement type="city">Düsseldorf</settlement></placeName></affiliation></author></analytic><series><title level="j">Proceedings. Biological sciences</title><idno type="eISSN">1471-2954</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biological Coevolution (MeSH)</term><term>Disease Resistance (genetics)</term><term>Lycopersicon esculentum (genetics)</term><term>Lycopersicon esculentum (microbiology)</term><term>MicroRNAs (genetics)</term><term>Phytophthora (physiology)</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (microbiology)</term><term>RNA, Plant (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN des plantes (génétique)</term><term>Coévolution biologique (MeSH)</term><term>Lycopersicon esculentum (génétique)</term><term>Lycopersicon esculentum (microbiologie)</term><term>Maladies des plantes (génétique)</term><term>Maladies des plantes (microbiologie)</term><term>Phytophthora (physiologie)</term><term>Résistance à la maladie (génétique)</term><term>microARN (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>MicroRNAs</term><term>RNA, Plant</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Disease Resistance</term><term>Lycopersicon esculentum</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN des plantes</term><term>Lycopersicon esculentum</term><term>Maladies des plantes</term><term>Résistance à la maladie</term><term>microARN</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Lycopersicon esculentum</term><term>Maladies des plantes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Lycopersicon esculentum</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biological Coevolution</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Coévolution biologique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Plants possess a battery of specific pathogen resistance (<i>R-</i>)genes. Precise <i>R-</i>gene regulation is important in the presence and absence of a pathogen. Recently, a microRNA family, miR482/2118, was shown to regulate the expression of a major class of <i>R-</i>genes<i>,</i> nucleotide-binding site leucine-rich repeats (<i>NBS-LRRs</i>). Furthermore, RNA silencing suppressor proteins, secreted by pathogens, prevent the accumulation of miR482/2118, leading to an upregulation of <i>R-</i>genes. Despite this transcriptional release of <i>R</i>-genes, RNA silencing suppressors positively contribute to the virulence of some pathogens<i>.</i> To investigate this paradox, we analysed how the regulation of <i>NBS-LRRs</i> by miR482/2118 has been shaped by the coevolution between <i>Phytophthora infestans</i> and cultivated and wild tomatoes. We used degradome analyses and qRT-PCR to evaluate and quantify the co-expression of miR482/2118 and their <i>NBS-LRR</i> targets. Our data show that miR482/2118-mediated targeting contributes to the regulation of <i>NBS-LRRs</i> in <i>Solanum lycopersicum.</i> Based on miR482/2118 expression profiling in two additional tomato species-with different coevolutionary histories with <i>P. infestans</i>-we hypothesize that pathogen-mediated RNA silencing suppression is most effective in the interaction between <i>S. lycopersicum</i> and <i>P. infestans</i> Furthermore, an upregulation of miR482/2118 early in the infection may increase susceptibility to <i>P. infestans</i>.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29491170</PMID><DateCompleted><Year>2019</Year><Month>01</Month><Day>04</Day></DateCompleted><DateRevised><Year>2019</Year><Month>01</Month><Day>30</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1471-2954</ISSN><JournalIssue CitedMedium="Internet"><Volume>285</Volume><Issue>1873</Issue><PubDate><Year>2018</Year><Month>02</Month><Day>28</Day></PubDate></JournalIssue><Title>Proceedings. Biological sciences</Title><ISOAbbreviation>Proc Biol Sci</ISOAbbreviation></Journal><ArticleTitle>Expression profiling across wild and cultivated tomatoes supports the relevance of early miR482/2118 suppression for <i>Phytophthora</i> resistance.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">20172560</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1098/rspb.2017.2560</ELocationID><Abstract><AbstractText>Plants possess a battery of specific pathogen resistance (<i>R-</i>)genes. Precise <i>R-</i>gene regulation is important in the presence and absence of a pathogen. Recently, a microRNA family, miR482/2118, was shown to regulate the expression of a major class of <i>R-</i>genes<i>,</i> nucleotide-binding site leucine-rich repeats (<i>NBS-LRRs</i>). Furthermore, RNA silencing suppressor proteins, secreted by pathogens, prevent the accumulation of miR482/2118, leading to an upregulation of <i>R-</i>genes. Despite this transcriptional release of <i>R</i>-genes, RNA silencing suppressors positively contribute to the virulence of some pathogens<i>.</i> To investigate this paradox, we analysed how the regulation of <i>NBS-LRRs</i> by miR482/2118 has been shaped by the coevolution between <i>Phytophthora infestans</i> and cultivated and wild tomatoes. We used degradome analyses and qRT-PCR to evaluate and quantify the co-expression of miR482/2118 and their <i>NBS-LRR</i> targets. Our data show that miR482/2118-mediated targeting contributes to the regulation of <i>NBS-LRRs</i> in <i>Solanum lycopersicum.</i> Based on miR482/2118 expression profiling in two additional tomato species-with different coevolutionary histories with <i>P. infestans</i>-we hypothesize that pathogen-mediated RNA silencing suppression is most effective in the interaction between <i>S. lycopersicum</i> and <i>P. infestans</i> Furthermore, an upregulation of miR482/2118 early in the infection may increase susceptibility to <i>P. infestans</i>.</AbstractText><CopyrightInformation>© 2018 The Authors.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>de Vries</LastName><ForeName>Sophie</ForeName><Initials>S</Initials><Identifier Source="ORCID">0000-0002-5267-8935</Identifier><AffiliationInfo><Affiliation>Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Canada NS B3H 4R2 sophie.devries@dal.ca.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Institute of Population Genetics, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kukuk</LastName><ForeName>Andreas</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Institute of Population Genetics, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>von Dahlen</LastName><ForeName>Janina K</ForeName><Initials>JK</Initials><AffiliationInfo><Affiliation>Institute of Population Genetics, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf, Germany.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>iGRAD-Plant Graduate School, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schnake</LastName><ForeName>Anika</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Institute of Population Genetics, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kloesges</LastName><ForeName>Thorsten</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Institute of Population Genetics, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rose</LastName><ForeName>Laura E</ForeName><Initials>LE</Initials><AffiliationInfo><Affiliation>Institute of Population Genetics, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf, Germany laura.rose@hhu.de.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>iGRAD-Plant Graduate School, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf, Germany.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Ceplas, Cluster of Excellence in Plant Sciences, Heinrich-Heine University Duesseldorf, Universitaetsstr. 1, 40225 Duesseldorf, Germany.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>figshare</DataBankName><AccessionNumberList><AccessionNumber>10.6084/m9.figshare.c.4002592</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Proc Biol 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first="Thorsten" last="Kloesges">Thorsten Kloesges</name><name sortKey="Kukuk, Andreas" sort="Kukuk, Andreas" uniqKey="Kukuk A" first="Andreas" last="Kukuk">Andreas Kukuk</name><name sortKey="Rose, Laura E" sort="Rose, Laura E" uniqKey="Rose L" first="Laura E" last="Rose">Laura E. Rose</name><name sortKey="Rose, Laura E" sort="Rose, Laura E" uniqKey="Rose L" first="Laura E" last="Rose">Laura E. Rose</name><name sortKey="Rose, Laura E" sort="Rose, Laura E" uniqKey="Rose L" first="Laura E" last="Rose">Laura E. Rose</name><name sortKey="Schnake, Anika" sort="Schnake, Anika" uniqKey="Schnake A" first="Anika" last="Schnake">Anika Schnake</name><name sortKey="Von Dahlen, Janina K" sort="Von Dahlen, Janina K" uniqKey="Von Dahlen J" first="Janina K" last="Von Dahlen">Janina K. Von Dahlen</name><name sortKey="Von Dahlen, Janina K" sort="Von Dahlen, Janina K" uniqKey="Von Dahlen J" first="Janina K" last="Von Dahlen">Janina K. Von Dahlen</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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